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Multiplate bioreactor for cell-based product manufacturing
Features and Benefits
- USP Class VI certified materials
- Designed for adherent cell culture applications
- Treatment for cells adherence
- Single-use closed bioreactor, from vessel to sensors, reduces risk of contamination
- 2-D multiplate design allows easy adaptation from traditional multitray systems
- Integrated,disposable pH DO probes
- Real-time control and monitoring ensures reproducibility and traceability
- Reduced operations results in process simplification, flexibility, and assurance
The Xpansion multiplate bioreactor is part of the Pall Life Sciences’ single-use bioreactor family, designed for shear-sensitive adherent cell applications, such as cultivation of stem cells.
The Xpansion multiplate bioreactor was developed for safe, large-scale production of traditional 2-D cell cultures.The multiplate structure is comprised of a large cell growth surface area (up to 122,400 cm2), while the compact design enables the elimination of the gas phase between the plates. This gas phase is replaced by an automatically controlled aeration system which provides advanced gas diffusion. Control is automatic via disposable pH and dissolved oxygen (DO) sensors. Temperature monitoring and agitation control are also included. Xpansion bioreactors offer the capability of monitoring cell morphology and density through the use of an optional digital holographic microscope.
Xpansion System Components
The Xpansion system includes a single-use bioreactor, docking station and a controller.
Single-Use Bioreactor with Stacked Multiplate DesignComprised of stacked hydrophilized polystyrene radial plates, the Xpansion bioreactor is designed to promote cell growth on the top of each plate; each plate has a hole in the center. The stacking of these plates forms the central column which allows circulation of the medium in the bioreactor and gas exchange.
The plates are covered by an outer tube, a head plate on the top and a base plate on the bottom. On the head plate, there are:- Six sensor connectors: pH, DO, and four optional sensors
- Three viewing windows for use with optional holographic microscope
- Two vent filters
- Two gas filters (in and out) for aeration system
- One thermowell port
- One sampling port
- Two fluid operation ports (e.g., inoculation, harvest, etc.)
- One magnetic impeller
An Xpansion Plate
The Xpansion Multiplate Bioreactor
1 | Gas lines (in & out) | C-Flex* tubing and silicone 3/16 in. ID, 0.2 μm hydrophobic filters (PTFE, polyester, polypropylene) |
2 | Vent.1 | C-Flex 3/16 in. ID, 2 0.2 μm hydrophobic filters (PTFE,polyester, polypropylene) |
3 | Sampling port | C-Flex 3/16 in. ID with needleless connector for sampling with luer syringe + oneway valve |
4 | Top part | Clear polystyrene |
5 | Cell surface plates | Cell culture plasma-treated clear polystyrene |
6 | Aeration support | Polypropylene + polycarbonate |
7 | Aeration tubing | Silicone 0.58 in. ID x 0.77 in. OD |
8 | Outer tube | Polyvinyl chloride |
9 | Stirrer cover | Clear polystyrene |
10 | Impeller | Ultra-high-molecular-weight polyethylene |
11 | Bearing | Polyether ether ketone |
12 | Bottom part | Clear polystyrene |
13 | Liquid in/out tubing | C-Flex Clear 374 Tubing 3/8 in. ID, CPC MPC male connector, 0.2 μm hydrophobic filters |
Sealing material | Resin | |
Docking Station
The bioreactor docks to a station that includes a magnetic drive stirring unit powering the magnetic impeller.
Control of agitation speed is integrated in the controller. The setpoint is defined via the screen on the front side of the controller.
Controller
The controller has measurement/control hardware and a gassing system with a digital control.
Features of the Xpansion controller include:- Graphic user interface with color display and touch screen operation with Administrator and Operator security levels
- Integrated transmitters for temperature, disposable DO and pH sensors
- Integrated control loops for DO and pH
- Agitation control (speed range from 5 to 250 rpm)
- Temperature monitoring
- DO and pH sensor patches arrive precalibrated; data are transferred via a USB key to the controller
- In-process DO and pH can be recalibrated
- Trend display for up to four process values (pH, DO, T, gas flow rate)
The controller is connected to the user’s gas supply line. Its “gas outlet” is connected to the “gas in” filter of the bioreactor. Gas composition to be injected in the bioreactor is regulated by controller solenoid gas valves. The flow rate of the mixed gas is adjusted by a massflow controller (0-200 mL/min). The controller gas module contains:
- One gas diffuser outlet
- Four solenoid valves of Air, N2, O2, and CO2
- One Mass Flow Controller for mixed gas flow control
The Xpansion system has one ethernet port for data communication/ acquisition. It can be linked to egraph (optional software and computer provided by Pall Life Sciences). egraph is installed on a host PC and provides data acquisition for all process values.
Xpansion Controller and Docking Station
Controller
Height | 50 cm (19.7 in.) |
Depth | 40 cm (15.7 in.) |
Width | 25 cm (9.8 in.) |
Weight | 15 kg (33 lbs.) |
Power supply | 100 – 240 VAC, 50 – 60 Hz |
Interface | Ethernet USB host |
Stirrer speed | 5 – 250 rpm |
Integrated measurement module | Disposable DO sensor Disposable pH sensor Temperature |
One point recalibration function | Disposable DO sensor Disposable pH sensor |
Gas options | O2 N2 CO2 Air |
Temperature monitoring | 0 – 50 ºC (32 – 122 ºF) |
Gas Requirements
Gases | Application | Specification |
Air | pH control | Compressed air – medical grade Outlet pressure between 1 and 2 bars (14.5 – 30 PSI) 6 mm Festo connectors |
CO2 | pH control | Pure CO2 Outlet pressure equivalent to air pressure 6 mm Festo connectors |
Oxygen | DO control | Medical grade oxygen Outlet pressure equivalent to air pressure 6 mm Festo connectors |
Nitrogen (optional) | DO control | Medical grade nitrogen Outlet pressure equivalent to air pressure 6 mm Festo connectors |
Specifications
Type
Use
Related Documents
Regulatory/Quality Documentation
Additional Information
Features and Benefits
Xpansion Bioreactor SystemScalable system |
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Same environment as multi-tray stack |
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Compact and closed system |
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Controlled environment |
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mPath Control Tower and mPath Link Server
mPath control tower designed for operation with mPath Link software - a SCADA software package |
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Ergonomic industrial design |
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See datasheet for documentation and certificates and Pall quality standards.
Protect Your Equipment and Optimize Productivity
Pall Corporation has over 70 years of experience in providing repair, calibration, maintenance, basic operator training, software upgrades, and other services to protect and maintain your equipment – all while optimizing your productivity workflow. Our expertise and short response-times ensure seamless operation, minimal downtime, and regulatory compliance throughout the lifetime of your equipment.
Our range of service packages keep your equipment protected and well maintained and include itemized pay-as you go services and start-up care packages to ease operator adoption in the first year of equipment ownership. Also available are post-warranty service plans containing tiered combinations of services to suit varied needs and offering added benefits such as comprehensive technical support, priority response time and discounts for emergency repairs, with flexible payment options. Our service plans provide total peace of mind and worry-free support throughout the coverage period.
For service support, contact Pall through service@pall.com (Americas), biotech_service_EMEA@pall.com (Europe and Africa), and APAC_Service@pall.com (Asia-Pacific) For technical support, contact the Pall technical hotline team through +1 (855) 920-7255 or NAHotline@pall.com (North America), and +800 7255 8324 or EMEAHotline@ pall.com (Europe and Africa)
Process Development ServicesPall AcceleratorSM process development services provide customers with expert advice and support for the implementation of fully integrated single-use processing solutions into new or existing processes, including process optimization, equipment selection, transfer of existing processes, scale-up and troubleshooting advice. Details can be found in the Accelerator Process Development Services brochure (USD 3079).
Our teams are specialized in upstream and downstream processing and will gladly help find the right technology for any part of your manufacturing process. It is possible to combine the Xpansion bioreactor system with other products to meet any requirement in the upstream part of the process:
- Media preparation
- Buffer preparation
- Media sterilization and aseptic transfer
- Liquid transfer into the bioreactor (e.g. glucose, anti-foam, base, etc.)
- Seed train solutions
- Cell harvest and separation
- Perfusion modules
Please contact Pall for a total solution discussion on your process.
Ordering Information
Description | Part Number |
Xpansion bioreactor system System hardware – consists of Xpansion hardware, mPath controller, control cables |
XPNBRS |
mPath Link server and SCADA software | MPATHLINK |
Harvest station Mechanical cell detachment device for harvest |
XPNHVST |
Lift Safe movement of bioreactor from package to incubator. Highly recommended for XP100 and XP200 |
XPNLIFT |
Consumables Xpansion 10 bioreactor Xpansion 50 bioreactor Xpansion 100 bioreactor Xpansion 200 bioreactor |
XPN10 XPN50 XPN100 XPN200 |
Seeding manifold 2 L bottle with female MPCu connector used to transfer cell suspension into seeding container |
6100097S |
Pooling manifold Sterile 10 L bottle with female MPC connector used for pooling reagent bottles |
6100129 |
Product Summary
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