AcroPrep™ Advance 96-Well Filter Plates for Ultrafiltration

Provides rapid, efficient separation of biomolecules

  • Omega™ membrane provides high recovery and typically results in > 90% recovery of target biomolecules
  • New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume for maximum sample recovery
  • Intrinsic plate and membrane properties prevent target molecules from binding to the plate
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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AcroPrep Advance Filter Plates for Ultrafiltration

Sorbent

Spare Parts and Accessories

Adapter collar for centrifugation (2/pkg)
Cap mat for incubation (5/pkg)
Description

Provides rapid, efficient separation of biomolecules

  • Omega™ membrane provides high recovery and typically results in > 90% recovery of target biomolecules
  • New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume for maximum sample recovery
  • Intrinsic plate and membrane properties prevent target molecules from binding to the plate
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Size exclusion
  • PCR clean-up
  • Nucleic acid purification
  • Protein separation
Specifications

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) membrane
  • Plate Housing: Polyproylene
  • Lid: Polystyrene

Dimensions

  • Length: 12.8 cm (5.0 in.)
  • Width: 8.6 cm (3.4 in.)
  • Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
  • Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.)

Well-Bottom Area

  • 0.25 cm2

Recommended Working Volume

  • 350 μL: < 300 μL
  • 1 mL: < 900 μL

Recommended Operating Vacuum

  • > 25.4 cm Hg (10 in. Hg)

Recommended Centrifugal Force

  • 1,500 x g

Typical Vacuum Filtration Performance

 
Membrane Processing Time (min) Hold-Up Volume (μL)
10K Omega 20 5
30K Omega 8 6
100K Omega 4 7
 

Typical Centrifugal Filtration Performance

 
Membrane Processing Time (min) Hold-Up Volume (μL)
3K Omega 45 2
10K Omega 8 2
30K Omega 8 2
100K Omega 5 2
 
Performance

Separation of Proteins with Omega Membrane Using Vacuum Filtration




SDS-PAGE analysis of reduced samples from 30K Omega membrane plate using vacuum separation method, 5 μL/lane. 75 μL protein mix/well subjected to ~20 in. Hg vacuum for ~8 minutes. Each protein at 100 μg/mL in initial mix. ML = Marker Ladder; M1 = BSA; M2 = BSA + Cyt C; M3 = BSA, Carbonic Anydrase, and Cyto C; M4 = Carbonic Anhydrase. GelCode Blue stain.
Type
Filter Plate
Use
Ultrafiltration, High throughput filtration