Typical Membrane Characteristics

Base Material	Grade	Thickness (mils)	Thickness (µm)
Asymmetric polysulfone	GF	12.99 +/- 0.79	330 +/- 20
Asymmetric polysulfone	GX	12.99 +/- 0.79	330 +/- 20
Asymmetric polysulfone	GR	12.99 +/- 0.79	330 +/- 20

Typical Performance Characteristics

Base Material	Grade	Blood Volume ( µ/cm2)	Membrane Void Volume	Plasma Separation Time (sec)	Plasma Recovery (%)	Applications
Asymmetric polysulfone	GF	20	1X	< 2 minutes	> 60	Small blood volume applications, such as finger sticks in formats like microfluidic and lateral flow POC devices. No post-treatment so material may exhibit higher hemolysis levels than other grades.
Asymmetric polysulfone	GX	20-30	1-1.5X	< 2 minutes	> 60	Small blood volume applications, such as finger sticks in formats like microfluidic and lateral flow POC devices. Also compatible with electrochemical analyte detection. Post-treatment helps to minimize hemolysis.
Asymmetric polysulfone	GR	40-50	2-2.5X	< 2 minutes	> 80	Large blood volume applications, such as lateral flow immunochromatographic devices. Post-treatment facilitates larger blood volumes with lower hemolysis.

Note: The product performance characteristics outlined above are determined using EDTA collected whole blood with typical hematocrit content of 45.6%. It is important to ensure that the membrane lies flat, that no end contact occurs, and to seal the edges by compression, etc. In addition, the underlying material has enough capillary force to wick the plasma from the membrane.

Note: The sample blood volume capacity of Vivid Plasma Separation membrane is defined as the amount of whole blood per cm² of medium that is rapidly and consistently separated (< 2 minutes) by the medium with low hemolysis. Blood volume capacity of the medium is directly related to its void volume (e.g., the space within the solid phase of the medium that is occupied by a liquid sample). The calculated void volume of Vivid Plasma Separation membrane is ~20 ± 1 µ/cm². With knowledge of the void volume within Vivid Plasma Separation membrane, and the specific membrane post-treatments utilized, we express recommended blood volume capacities that generate rapid and consistent plasma separation with minimal hemolysis. It is essential that these blood sample volume recommendations are followed in order to achieve optimal conditions for the preparation of quality plasma.

Applications

• Plasma generation from whole blood
• Lateral flow diagnostics

Sealing

• Mechanical
• Adhesive
• Lamination

Achieve High Plasma Yields with Vivid Plasma Separation Membrane

Plasma recovery (in percent of available plasma) is presented as orange columns (values are to be read at the left Y-axis) against the volumes of blood applied to the Vivid Plasma Separation GR membrane (shown on X-axis in void volume increments; one void volume is equal to 20 µ of blood). One can see that the percent of plasma recovered from different volumes of blood does not depend on the blood volume applied to the media. Separation times are plotted as teal rhombs with values read at the right Y-axis. The graphs show that the separation time increases dramatically with increasing blood volume applied to the media.

Low Hemolysis with Vivid Plasma Separation Membrane

A 47 mm disc of Vivid Plasma Separation Membrane was placed flat on the bottom of a small petri dish. 600 µ of fresh whole EDTA blood was applied to the disc and separation was completed in approximately 6 minutes. Each disc was carefully slid along the bottom of the petri dish to access the plasma. Plasma was pooled at the edge of the dish and used for immediate total protein, cell, and hemoglobin measurements. The resulting plasma has been demonstrated as cell and hemolysis free.

Vivid Plasma Separation Membrane Does Not Bind Clinically Relevant Protein Biomarkers from Plasma Samples

Troponin I concentration was measured in plasma samples filtered through Vivid Plasma Separation membrane (blue columns) versus control centrifuged plasma (orange column). All plasma samples were generated from the same sample of fresh EDTA blood spiked with Troponin I at 1 ng/mL. Protein concentration in each sample was measured in triplicate.

No Significant Difference in the Protein Profiles of Vivid Plasma Separation Filtered Plasma and Control Centrifuged Plasma

A 2DE protocol of very high resolution of the acidic pI, medium molecular weight region where most of the known cardiac biomarkers are located, employed a first dimension with pH 4-7 NL IPG strips followed by second dimension separation on 10.5-14% SDS PAGE. 2DE profiles of control centrifuged plasma (Picture A) and Vivid Plasma Separation membrane separated plasma (Pictures B and C) are presented. There is no significant difference between the Vivid Plasma Separation separated plasma and the centrifuged control plasma.

Membrane Filters

Diagnostics, Separation

Custom roll, sheet, and disc sizes available upon request. Please contact your local sales representative for additional information.

Part Number	Description	Pkg
T9EXPPA0200S00A	Vivid Plasma Separation GF membrane, 8" x 11" sheet	1/pkg
T9EXPPA0200S00X	Vivid Plasma Separation GX membrane, 8" x 11" sheet	1/pkg
T9EXPPA0200S00R	Vivid Plasma Separation GR membrane, 8" x 11" sheet	1/pkg

• White Blood Cell Isolation Medium isolates leukocytes from whole blood samples. The nucleic acid content can be extracted for further analysis in molecular detection applications.