For Biomolecule Detection

           

Nylon membranes from Pall Corporation have been recognized as the gold standard for nucleic acid detection for more than 20 years. Our nylon membranes offer unsurpassed sensitivity, low background, and lot-to-lot consistency for all radioactive and non-radioactive detection methods. The intrinsically hydrophilic nature of our nylon membranes result in easy wetting across the membrane. When subjected to multiple cycles of hybridization, stripping and reprobing, the stability and durability of our nylon membranes is showcased as they will not crack, shrink, or tear.

We have five chemistries available that provide versatile adsorption properties.

Amphoteric Nylon 6,6

The net charge of this membrane can be modulated by changes in pH. It is ideal for single probe or multiple rehybridizations and in applications where background is troublesome. The binding mechanism for this membrane is through hydrophobic and electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking. It is well suited for nucleic acid dot blots, gene probe assays, and DNA fingerprinting.

 

Positively-Charged Nylon 6,6

The pore surfaces of this membrane are populated by a high density of quarternary ammonium groups. It offers high sensitivity in nucleic acid detection applications. The binding mechanism for this membrane is through electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking although it is not required. It is well suited for nucleic acid dot blots, DNA fingerprinting, and colony/plaque lifts.

 

Positively-Charged Nylon 6,6 with High Isoelectric Point

This membrane has an extremely high isoelectric point which provides greater sensitivity than the amphoteric nylon while exhibiting lower background than the standard positively-charged nylon membrane in certain non-radioactive detection systems. It is our most sensitive nylon membrane for nucleic acid detection. The binding mechanism for this membrane is through electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking although it is not required. It is well suited for nucleic acid dot blots
and DNA fingerprinting.

Negatively-Charged Nylon 6,6

The surface of this membrane is covered with a high density of carboxyl groups that can be derivatized for protein attachment. The binding mechanism for this membrane is through electrostatic interactions. It is well suited for protein immobilization, ELISAs, and affinity purification via ligand attachment.

 

Modified Nylon 6,6

This membrane is surface modified through proprietary chemistry which allows for covalent binding and stable ligand immobilization. The membrane is preactivated to form covalent linkages with nucleophilic groups found on proteins and other biological macromolecules. Primary reactivity is with amine groups at neutral pH. The microporous structure of the membrane provides an available immobilization area of up to 300 cm² for each cm² of planar membrane. It is intrinsically hydrophilic and exhibits instantaneous wetting on contact with low ionic strength aqueous solutions. High capillarity and rapid absorbent wicking are obtainable without the necessity of surface modifying wetting agents. Prewetting is not required prior to contact with ligand solutions. This membrane offers greater binding capacity for proteins than traditional non-porous solid phase surfaces.